Alternative titles; symbols
HGNC Approved Gene Symbol: TWF2
Cytogenetic location: 3p21.2 Genomic coordinates (GRCh38) : 3:52,228,612-52,239,158 (from NCBI)
Using the catalytic domain of rat Pkc-zeta (176982) as bait in a yeast 2-hybrid screen, Rohwer et al. (1999) cloned PTK9L, which they called A6RP, from a human keratinocyte cell line cDNA library. The deduced 349-amino acid protein has a calculated molecular mass of 39.5 kD. PTK9L contains a consensus sequence for actin-depolymerizing proteins and 2 ATP-binding motifs. It shares 64% amino acid identity with the human A6 protein (PTK9), 97% identity with mouse Ptk9l, 34% identity with C. elegans A6, and 26% identity with yeast twinfilin (see 610932). RT-PCR and Western blot analysis detected PTK9L in all human and mouse tissues and cells tested. Immunofluorescence localization revealed spotty accumulation of PTK9L in the cytoplasm close to nuclei.
Rohwer et al. (1999) found that PTK9L was phosphorylated by PKC-zeta, but not significantly by other PKC isoenzymes. It was also phosphorylated by casein kinase II (see 115442) and most effectively by the tyrosine kinase SRC (190090). In accordance with its potential ATP-binding site, PTK9L was able to bind ATP.
The International Radiation Hybrid Mapping Consortium mapped the PTK9L gene to chromosome 3 (stSG1676).
Rohwer, A., Kittstein, W., Marks, F., Gschwendt, M. Cloning, expression and characterization of an A6-related protein. Europ. J. Biochem. 263: 518-525, 1999. [PubMed: 10406962] [Full Text: https://doi.org/10.1046/j.1432-1327.1999.00537.x]