HGNC Approved Gene Symbol: ELSPBP1
Cytogenetic location: 19q13.33 Genomic coordinates (GRCh38) : 19:47,994,632-48,025,154 (from NCBI)
Saalmann et al. (2001) cloned Elspbp1 from canine epididymis by differential display, and used this sequence to clone human ELSPBP1 from an epididymal cDNA library. The deduced 233-amino acid protein contains an N-terminal signal peptide characteristic of a secretory protein, and 4 tandem fibronectin type II (FN2) repeats. RT-PCR of epididymal cDNA suggested the presence of splice variants containing fewer FN2 modules. Human and canine proteins share nearly 80% identity. Northern blot analyses of both human and canine tissues revealed high levels of a 1.1-kb ELSPBP1 transcript only in epididymis, and not in other tissues investigated, including human testis, seminal vesicles, and prostate. Northern blot analysis of dissected human epididymis indicated expression in all regions, with highest expression in the corpus. In situ localization of Elspbp1 in canine epididymis indicated high expression along the basal surface of the duct epithelium, with strongest labeling of the distal caput and corpus epididymis. Western blot analysis revealed a protein of 30 to 35 kD in human epididymal fluid and in membrane protein preparations of ejaculated sperm.
Saalmann, A., Munz, S., Ellerbrock, K., Ivell, R., Kirchhoff, C. Novel sperm-binding proteins of epididymal origin contain four fibronectin type II-modules. Molec. Reprod. Dev. 58: 88-100, 2001. [PubMed: 11144225] [Full Text: https://doi.org/10.1002/1098-2795(200101)58:1<88::AID-MRD12>3.0.CO;2-D]