Alternative titles; symbols
HGNC Approved Gene Symbol: RYBP
Cytogenetic location: 3p13 Genomic coordinates (GRCh38) : 3:72,374,597-72,446,623 (from NCBI)
Using mouse Ring1 (602045) as bait in a yeast 2-hybrid screen, Garcia et al. (1999) cloned Rybp from a mouse embryo cDNA library. The deduced 227-amino acid protein has an N-terminal C2C2 zinc finger motif and shows blocks of sequence identity with human YAF2 (607534) ranging from about 80% in the zinc finger domains to about 45% at the C termini.
Using the non-DNA-binding subunit of GABPB (600610) as bait in a yeast 2-hybrid screen, Sawa et al. (2002) cloned human RYBP, which they called YEAF1, from a HeLa cell cDNA library. Northern blot analysis detected ubiquitous expression of a 5-kb RYBP transcript, with highest expression in placenta.
Using in vitro binding assays, Garcia et al. (1999) determined that mouse Rybp interacts with the M33 (CBX2; 602770)-binding domain of Ring1. They found that Rybp also directly interacts with a domain of M33 that is not involved in Ring1 binding. Garcia et al. (1999) determined that mouse Rybp acts as a transcriptional repressor in transiently transfected cells. During mouse embryogenesis, expression of Rybp initially overlapped that of Ring1 in the central nervous system and later became ubiquitous.
Sawa et al. (2002) found that both RYBP and YAF2 interacted with GABPB and YY1 (600013) in vitro and in vivo. Using a yeast 3-hybrid assay, they demonstrated that GABPB and YY1 formed a complex only in the presence of RYBP. They also determined that RYBP and YAF2 are functionally different in that YAF2 activates, and RYBP represses, the transcriptional activity of GABP.
Hartz (2013) mapped the RYBP gene to chromosome 3p13 based on an alignment of the RYBP sequence (GenBank AB029551) with the genomic sequence (GRCh37).
Hisada et al. (2012) stated that Rybp knockout in mice results in developmental arrest around gastrulation and that embryonic stem (ES) cells cannot be established from early Rybp-knockout embryos. Using a conditional deletion approach, Hisada et al. (2012) found that Rybp was not required for maintenance of mouse ES cells. However, expression profiling revealed that Rybp was required to repress specific endogenous retroviruses and preimplantation- and germline-specific genes. Rybp had only modest effect on Polycomb group genes (see 610231). Chromatin immunoprecipitation analysis revealed that Rybp bound to chromatin showing repressive marks, but binding did not always correlate with Rybp-dependent repression.
Garcia, E., Marcos-Gutierrez, C., del Mar Lorente, M., Moreno, J. C., Vidal, M. RYBP, a new repressor protein that interacts with components of the mammalian Polycomb complex, and with the transcription factor YY1. EMBO J. 18: 3404-3418, 1999. [PubMed: 10369680] [Full Text: https://doi.org/10.1093/emboj/18.12.3404]
Hartz, P. A. Personal Communication. Baltimore, Md. 1/14/2013.
Hisada, K., Sanchez, C., Endo, T. A., Endoh, M., Roman-Trufero, M., Sharif, J., Koseki, H., Vidal, M. RYBP represses endogenous retroviruses and preimplantation- and germ line-specific genes in mouse embryonic stem cells. Molec. Cell. Biol. 32: 1139-1149, 2012. [PubMed: 22269950] [Full Text: https://doi.org/10.1128/MCB.06441-11]
Sawa, C., Yoshikawa, T., Matsuda-Suzuki, F., Delehouzee, S., Goto, M., Watanabe, H., Sawada, J., Kataoka, K., Handa, H. YEAF1/RYBP and YAF-2 are functionally distinct members of a cofactor family for the YY1 and E4TF1/hGABP transcription factors. J. Biol. Chem. 277: 22484-22490, 2002. [PubMed: 11953439] [Full Text: https://doi.org/10.1074/jbc.M203060200]