Alternative titles; symbols
HGNC Approved Gene Symbol: PAQR7
Cytogenetic location: 1p36.11 Genomic coordinates (GRCh38) : 1:25,861,484-25,875,708 (from NCBI)
Using homology with the sequence of seatrout membrane progestin receptor, Zhu et al. (2003) identified ESTs containing MPRA, and they cloned full-length cDNAs from several vertebrate species, including human. The deduced human, pig, and mouse MPRA proteins contain 346 to 350 amino acids, consisting of an extracellular N terminus, an intracellular C terminus, and 7 transmembrane domains. Northern blot analysis detected a major 1.4-kb transcript and a minor 2.5-kb transcript expressed at high levels only in testis. RNA dot blot analysis detected highest expression in placenta, testis, and ovary, followed by uterus, bladder, kidney, and possibly adrenal gland.
Tang et al. (2005) stated that the 7-transmembrane domain of the full-length 346-amino acid PAQR7 protein shares significant similarity with those of ADIPOR1 (607945) and ADIPOR2 (607946). The mouse ortholog shares 82% amino acid identity with human PAQR7. RT-PCR detected robust PAQR7 expression in all 20 human tissues examined, with highest expression in adrenal gland, testis, and skin.
Zhu et al. (2003) determined that recombinant MPRA, expressed by E. coli, showed high-affinity progesterone binding.
Using microarray analysis, Yu et al. (2004) found that Pglp was among the genes upregulated in a mouse model of hepatic steatosis caused by Pparg (601487) overexpression on a Ppara (170998) -/- background. Pglp expression was not upregulated in hepatic steatosis induced by fasting or choline deficiency.
Tang et al. (2005) determined that the PAQR7 gene contains a single coding exon.
Zhu (2003) stated that the MPRA gene maps to chromosome 1. By genomic sequence analysis, Tang et al. (2005) mapped the PAQR7 gene to chromosome 1p36.11.
Tang, Y. T., Hu, T., Arterburn, M., Boyle, B., Bright, J. M., Emtage, P. C., Funk, W. D. PAQR proteins: a novel membrane receptor family defined by an ancient 7-transmembrane pass motif. J. Molec. Evol. 61: 372-380, 2005. [PubMed: 16044242] [Full Text: https://doi.org/10.1007/s00239-004-0375-2]
Yu, S., Viswakarma, N., Batra, S. K., Rao, M. S., Reddy, J. K. Identification of promethin and PGLP as two novel up-regulated genes in PPAR-gamma-1-induced adipogenic mouse liver. Biochimie 86: 743-761, 2004. [PubMed: 15589683] [Full Text: https://doi.org/10.1016/j.biochi.2004.09.015]
Zhu, Y. Personal Communication. Baltimore, Md. 4/25/2003.
Zhu, Y., Bond, J., Thomas, P. Identification, classification, and partial characterization of genes in humans and other vertebrates homologous to a fish membrane progestin receptor. Proc. Nat. Acad. Sci. 100: 2237-2242, 2003. [PubMed: 12601167] [Full Text: https://doi.org/10.1073/pnas.0436133100]