Alternative titles; symbols
HGNC Approved Gene Symbol: PAQR5
Cytogenetic location: 15q23 Genomic coordinates (GRCh38) : 15:69,298,912-69,407,780 (from NCBI)
Using homology with the sequence of seatrout membrane progestin receptor, Zhu et al. (2003) identified ESTs containing MPRG, and they cloned full-length human, mouse, and pufferfish MPRG cDNAs. The deduced proteins contain about 330 amino acids, consisting of an extracellular N terminus, an intracellular C terminus, and 7 transmembrane domains. Northern blot analysis detected a 5.8-kb transcript expressed at high levels only in kidney. RNA dot blot analysis detected highest expression in adult and fetal kidney, colon, lung carcinoma, and HeLa cells, and showed possible expression in adrenal gland and normal lung.
Tang et al. (2005) stated that the 7-transmembrane domain of the full-length 330-amino acid PAQR5 protein shares significant similarity with those of ADIPOR1 (607945) and ADIPOR2 (607946). The mouse ortholog shares 91% amino acid identity with human PAQR5. RT-PCR detected highest PAQR5 expression in human lung, colon, and small intestine. Weaker expression was detected in pancreas, lymph node, kidney, brain, testis, bladder, skin, and adult bone marrow, and little to none was detected in adrenal gland, stomach, skeletal muscle, adipose, liver, prostate, breast, heart, and thymus.
Zhu et al. (2003) determined that recombinant MPRG, expressed by E. coli, showed high-affinity progesterone binding. Steroid competition studies showed specific binding to progesterone and several of its hydroxylated derivatives, whereas estrogen, androgen, and corticosteroid had low or no affinity for the receptor. Association and dissociation kinetics were rapid.
Tang et al. (2005) determined that the PAQR5 gene contains 7 coding exons.
Zhu (2003) stated that the human MPRG gene maps to chromosome 15. By genomic sequence analysis, Tang et al. (2005) mapped the PAQR5 gene to chromosome 15q23.
Tang, Y. T., Hu, T., Arterburn, M., Boyle, B., Bright, J. M., Emtage, P. C., Funk, W. D. PAQR proteins: a novel membrane receptor family defined by an ancient 7-transmembrane pass motif. J. Molec. Evol. 61: 372-380, 2005. [PubMed: 16044242] [Full Text: https://doi.org/10.1007/s00239-004-0375-2]
Zhu, Y. Personal Communication. Baltimore, Md. 4/25/2003.
Zhu, Y., Bond, J., Thomas, P. Identification, classification, and partial characterization of genes in humans and other vertebrates homologous to a fish membrane progestin receptor. Proc. Nat. Acad. Sci. 100: 2237-2242, 2003. [PubMed: 12601167] [Full Text: https://doi.org/10.1073/pnas.0436133100]