HGNC Approved Gene Symbol: CLK4
Cytogenetic location: 5q35.3 Genomic coordinates (GRCh38) : 5:178,602,664-178,627,050 (from NCBI)
By searching an EST database for CDC-like kinases, followed by RT-PCR of total kidney RNA, Schultz et al. (2001) cloned CLK4. The deduced 481-amino acid protein has a calculated molecular mass of about 54 kD. CLK4 shares 97% identity with murine Clk4. Northern blot analysis detected 4 transcripts that ranged from about 2.0 to 4.5 kb. Strongest expression was in liver, kidney, heart, skeletal muscle, and brain. All 4 transcripts were expressed evenly in kidney and liver, while the 2.8-kb transcript predominated in heart and skeletal muscle. Analysis of a multiple tissue expression array showed CLK4 expression in all samples tested.
Schultz et al. (2001) demonstrated that recombinant CLK4 expressed in E. coli phosphorylated myelin basic protein (159430) and autophosphorylated, but it did not phosphorylate H2B (see 609904). CLK4 containing a lys189-to-arg mutation was catalytically inactive.
By FISH, Schultz et al. (2001) mapped the CLK4 gene to chromosome 5q35.
Schultz, J., Jones, T., Bork, P., Sheer, D., Blencke, S., Steyrer, S., Wellbrock, U., Bevec, D., Ullrich, A., Wallasch, C. Molecular characterization of a cDNA encoding functional human CLK4 kinase and localization to chromosome 4q35. Genomics 71: 368-370, 2001. Note: Erratum: Genomics 74: 251 only, 2001. [PubMed: 11170754] [Full Text: https://doi.org/10.1006/geno.2000.6447]