Alternative titles; symbols
HGNC Approved Gene Symbol: ACRBP
Cytogenetic location: 12p13.31 Genomic coordinates (GRCh38) : 12:6,638,075-6,647,432 (from NCBI)
By searching an EST database for sequences similar to mouse Pem (see 300446), followed by PCR and 5-prime RACE of a human testis cDNA library, Ono et al. (2001) cloned ACRBP, which they designated OY-TES-1. The deduced 543-amino acid protein contains an N-terminal glutamic acid- and glutamine-rich domain and a central acidic amino acid region. It has 20 cysteine residues that are completely conserved between several mammalian species. The mature protein is cleaved between arg273 and glu274. ACRBP shares 77.2%, 81.9%, and 75.2% amino acid identity with its guinea pig, pig, and mouse homologs, respectively. RT-PCR of several human tissues detected ACRBP expression only in testis. ACRBP was also expressed in a subset of tumors of varied tissue origin. A serologic survey found antibodies against ACRBP in 25 of 362 cancer patients. No ACRBP reactivity was found in 20 normal individuals.
Ono et al. (2001) determined that the ACRBP gene contains 10 exons and spans more than 9.3 kb.
By radiation hybrid analysis and FISH, Ono et al. (2001) mapped the ACRBP gene to chromosome 12p13-p12. Southern blot analysis indicated that there are 2 ACRBP-related genes in the human genome.
Ono, T., Kurashige, T., Harada, N., Noguchi, Y., Saika, T., Niikawa, N., Aoe, M., Nakamura, S., Higashi, T., Hiraki, A., Wada, H., Kumon, H., Old, L. J., Nakayama, E. Identification of proacrosin binding protein sp32 precursor as a human cancer/testis antigen. Proc. Nat. Acad. Sci. 98: 3282-3287, 2001. [PubMed: 11248070] [Full Text: https://doi.org/10.1073/pnas.041625098]