Alternative titles; symbols
HGNC Approved Gene Symbol: TADA2B
Cytogenetic location: 4p16.1 Genomic coordinates (GRCh38) : 4:7,043,372-7,057,952 (from NCBI)
TADA2B functions as a transcriptional adaptor protein that potentiates transcription through coordination of histone acetyltransferase (HAT) activity and by linking activation factors to basal transcriptional machinery (Barlev et al., 2003).
To identify additional proteins that interact with PCAF (KAT2B; 602303)- and GCN5 (KAT2A; 602301)-containing HAT complexes, Barlev et al. (2003) screened a mouse embryonic cDNA library using PCAF as bait and identified mouse Ada2b. They cloned human ADA2B from osteosarcoma cells using RT-PCR. The predicted 420-amino acid human protein shares 100% identity with mouse Ada2b and 59% similarity with human ADA2A (TADA2A; 602276). Database analysis identified ADA2B homologs in Drosophila and Arabidopsis. Western blot analysis detected a 55- to 62-kD ADA2B protein in the nuclear fraction of HeLa cells.
Barlev et al. (2003) found that ADA2B did not coimmunoprecipitate with the PCAF complex, but it did interact with PCAF, GCN5, and the chromatin remodeling Swi/Snf complex protein BAF57 (603111) in yeast 2-hybrid analyses. Several assays showed that ADA2B associated with BAF57-/BRG1 (603254)-containing complexes in vivo. Barlev et al. (2003) demonstrated that ADA2B, together with GCN5 or BRG1, interacted with PAX5 (167414) and coactivated PAX5-dependent transcription.
Using mass spectrometry, Wang et al. (2008) identified ADA2B as a subunit of the SPT3 (SUPT3H; 602947)-TAF9 (600822)-GCN5/PCAF acetylase (STAGA) complex. It did not purify with components of the ADA2A-containing (ATAC) HAT complex, which also includes GCN5 or PCAF.
Barlev, N. A., Emelyanov, A. V., Castagnino, P., Zegerman, P., Bannister, A. J., Sepulveda, M. A., Robert, F., Tora, L., Kouzarides, T., Birshtein, B. K., Berger, S. L. A novel human Ada2 homologue functions with Gcn5 or Brg1 to coactivate transcription. Molec. Cell. Biol. 23: 6944-6957, 2003. [PubMed: 12972612] [Full Text: https://doi.org/10.1128/MCB.23.19.6944-6957.2003]
Wang, Y.-L., Faiola, F., Xu, M., Pan, S., Martinez, E. Human ATAC is a GCN5/PCAF-containing acetylase complex with a novel NC2-like histone fold module that interacts with the TATA-binding protein. J. Biol. Chem. 283: 33808-33815, 2008. [PubMed: 18838386] [Full Text: https://doi.org/10.1074/jbc.M806936200]