Alternative titles; symbols
HGNC Approved Gene Symbol: ZNF330
Cytogenetic location: 4q31.21 Genomic coordinates (GRCh38) : 4:141,220,535-141,234,697 (from NCBI)
Using a human autoimmune serum against the nucleolar organizer region (NOR), Bolivar et al. (1999) isolated a hamster cDNA that they called NOA36. They used this cDNA as a probe to select human ZNF330 cDNAs from a HeLa cell library. The human ZNF330 gene encodes a 320-amino acid protein with a predicted molecular mass of 36.3 kD. The ZNF330 protein contains a putative nuclear localization signal, 9 conserved CXXC zinc finger motifs, and an acidic C terminus. EST analysis identified ZNF330 homologs in hamster, mouse, Drosophila, and nematode. Northern blot analysis showed expression of a 1.8-kb ZNF330 transcript in all tissues examined, with highest expression seen in heart and skeletal muscle.
By Western blot analysis using recombinant polypeptides and by immunofluorescence studies, Bolivar et al. (1999) confirmed that ZNF330 is recognized by anti-NOR serum. Further immunofluorescence studies in mammalian cells demonstrated that ZNF330 localizes primarily to the nucleolus during interphase and to centromeres during mitosis. They also showed that after inhibition of transcription by actinomycin-D treatment, ZNF330 remains associated with the nucleolus, but no longer colocalizes with the nucleolar factor UBTF (600673).
Using RNAse treatment, Bolivar et al. (2001) showed that the nucleolar association of ZNF330 is RNA-dependent.
Bolivar et al. (2001) showed that the ZNF330 gene contains 10 exons and spans 16 kb.
Using FISH and genomic database analyses, Bolivar et al. (2001) mapped the ZNF330 gene to 4q31.1-q31.2.
Bolivar, J., Diaz, I., Iglesias, C., Valdivia, M. M. Molecular cloning of a zinc finger autoantigen transiently associated with interphase nucleolus and mitotic centromeres and midbodies: orthologous proteins with nine CXXC motifs highly conserved from nematodes to humans. J. Biol. Chem. 274: 36456-36464, 1999. [PubMed: 10593942] [Full Text: https://doi.org/10.1074/jbc.274.51.36456]
Bolivar, J., Garcia-Cozar, F. J., Astola, A., Iglesias, C., Pendon, C., Valdivia, M. M. Genomic structure and chromosome location of the human gene encoding the zinc finger autoantigen ZNF330. Cytogenet. Cell Genet. 93: 234-238, 2001. [PubMed: 11528117] [Full Text: https://doi.org/10.1159/000056989]