Alternative titles; symbols
HGNC Approved Gene Symbol: PDZRN3
Cytogenetic location: 3p13 Genomic coordinates (GRCh38) : 3:73,382,431-73,624,941 (from NCBI)
By sequencing clones obtained from an adult brain cDNA library, Kikuno et al. (1999) cloned PDZRN3, which they designated KIAA1095. The deduced 1,098-amino acid protein contains a C3HC4 zinc finger motif. RT-PCR ELISA detected high expression in heart, whole brain, lung, skeletal muscle, ovary, spinal cord, thalamus, amygdala, and fetal whole brain. Lower expression was detected in kidney, testis, pancreas, adult and fetal liver, and all other specific brain regions tested. Lowest expression was detected in spleen.
By searching for genes encoding proteins structurally similar to LNX (LNX1; 609732) and PDZRN1 (LNX2; 609733), Katoh and Katoh (2004) identified PDZRN3. The deduced 1,066-amino acid protein contains an N-terminal RING domain, followed by 2 PDZ domains and 2 C-terminal bipartite nuclear localization signals. PDZRN3 shares 59.9% amino acid identity with PDZRN4 (609730).
Mesenchymal progenitor cells, such as mouse C2C12 cells, can differentiate into osteoblasts, chondrocytes, adipocytes, or myotubes. Honda et al. (2010) found that osteoblastic differentiation of C2C12 cells induced by human BMP2 (112261) was accompanied by an early increase in Pdzrn3 expression. Furthermore, BMP2-mediated differentiation was enhanced by knockdown of Pdzrn3 via RNA interference. Examination of the signaling pathways involved suggested that Pdzrn3 countered BMP2-mediated differentiation by inhibition of Wnt (see WNT3A; 606359)-beta-catenin (CTNNB1; 116806) signaling. Pdzrn3 suppressed both Lrp6 (603507) expression and Wnt3a-induced phosphorylation of Lrp6, which appeared to be critical for negative regulation of osteoblastic differentiation by Pdzrn3.
Katoh and Katoh (2004) determined that the PDZRN3 gene contains 10 exons.
By genomic sequence analysis, Katoh and Katoh (2004) mapped the PDZRN3 gene to chromosome 3p13.
Honda, T., Yamamoto, H., Ishii, A., Inui, M. PDZRN3 negatively regulates BMP-2-induced osteoblast differentiation through inhibition of Wnt signaling. Molec. Biol. Cell 21: 3269-3277, 2010. [PubMed: 20668165] [Full Text: https://doi.org/10.1091/mbc.E10-02-0117]
Katoh, M., Katoh, M. Identification and characterization of PDZRN3 and PDZRN4 genes in silico. Int. J. Molec. Med. 13: 607-613, 2004. [PubMed: 15010864]
Kikuno, R., Nagase, T., Ishikawa, K., Hirosawa, M., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 6: 197-205, 1999. [PubMed: 10470851] [Full Text: https://doi.org/10.1093/dnares/6.3.197]