Alternative titles; symbols
HGNC Approved Gene Symbol: PRAC1
Cytogenetic location: 17q21.32 Genomic coordinates (GRCh38) : 17:48,721,719-48,722,518 (from NCBI)
By in silico analysis, Liu et al. (2001) identified a cluster of sequence-homologous ESTs derived only from human prostate cDNA libraries. Using these EST sequences and 5-prime RACE, they assembled a full-length cDNA, which they designated PRAC (prostate, rectum, and colon), from LNCaP mRNA and prostate tumor total RNA. PRAC encodes a deduced 57-amino acid protein with several potential phosphorylation sites and a molecular mass of about 6 kD. RNA dot-blot analysis detected high expression of PRAC in prostate, rectum, and distal colon, and weak expression in bladder. No difference in expression was found between normal prostate and prostate tumor samples. Northern blot analysis revealed a 450-bp PRAC transcript in prostate and colon as well as in the prostate adenocarcinoma cell lines LNCaP and PC-3. RT-PCR detected low expression in the prostate cell line DU145. Western blot analysis localized PRAC to the nucleus.
By sequence analysis, Liu et al. (2001) mapped the PRAC1 gene to chromosome 17q21.3 about 4 kb downstream from the HOXB13 gene (604607).
Liu, X. F., Olsson, P., Wolfgang, C. D., Bera, T. K., Duray, P., Lee, B., Pastan, P. PRAC: a novel small nuclear protein that is specifically expressed in human prostate and colon. Prostate 47: 125-131, 2001. [PubMed: 11340635] [Full Text: https://doi.org/10.1002/pros.1055]