Alternative titles; symbols
HGNC Approved Gene Symbol: CAND2
Cytogenetic location: 3p25.2 Genomic coordinates (GRCh38) : 3:12,796,680-12,834,803 (from NCBI)
By sequencing clones obtained from a size-fractionated brain cDNA library, Ishikawa et al. (1998) cloned CAND2, which they designated KIAA0667. The deduced protein shares significant similarity with rat Tip120 (CAND1; 607727). RT-PCR detected highest CAND2 expression in skeletal muscle, intermediate expression in heart and testis, low expression in brain, ovary, and lung, and very low expression in all other tissues examined.
By 5-prime RACE, Aoki et al. (1999) identified 2 different forms of mouse and rat Cand2, which they called Tip120b. Northern blot analysis of several rat tissues detected expression only in skeletal muscle and heart. In mouse embryos, Tip120b expression began at embryonic day 11, a stage that coincides with limb bud formation, then expression decreased until day 17. Western blot analysis detected a 135-kD protein in rat skeletal muscle, heart, and testis.
Using a pull-down assay, Aoki et al. (1999) demonstrated that TATA-binding protein (TBP; 600075) interacted with Tip120b in cultured mouse myotube nuclear extracts.
By radiation hybrid analysis, Ishikawa et al. (1998) mapped the CAND2 gene to chromosome 3.
Aoki, T., Okada, N., Ishida, M., Yugosawa, S., Makino, Y., Tamura, T. TIP120B: a novel TIP120-family protein that is expressed specifically in muscle tissues. Biochem. Biophys. Res. Commun. 261: 911-916, 1999. [PubMed: 10441524] [Full Text: https://doi.org/10.1006/bbrc.1999.1147]
Ishikawa, K., Nagase, T., Suyama, M., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. DNA Res. 5: 169-176, 1998. [PubMed: 9734811] [Full Text: https://doi.org/10.1093/dnares/5.3.169]