Alternative titles; symbols
HGNC Approved Gene Symbol: ANKRD12
Cytogenetic location: 18p11.22 Genomic coordinates (GRCh38) : 18:9,136,781-9,285,985 (from NCBI)
By sequencing clones obtained from a size-fractionated human brain cDNA library, Nagase et al. (1998) cloned ANKRD12, which they designated KIAA0874. The deduced protein contains 601 amino acids. RT-PCR ELISA detected moderate expression in brain, kidney, heart, lung, liver, testis, and ovary. Low expression was detected in skeletal muscle, pancreas, and spleen.
Using the p19(Arf) isoform of mouse Cdkn2a (600160) as bait in a yeast 2-hybrid screen of a mouse embryonic fibroblast cDNA library, Tompkins et al. (2006) cloned Ankrd12. The deduced protein contains an ankyrin repeat. Tompkins et al. (2006) referred to Ankrd12 as Anco1.
Using an in vitro binding assay, Tompkins et al. (2006) confirmed binding between mouse p19(Arf) and Ankrd12.
By radiation hybrid analysis, Nagase et al. (1998) mapped the ANKRD12 gene to chromosome 18.
Nagase, T., Ishikawa, K., Suyama, M., Kikuno, R., Hirosawa, M., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Oharo, O. Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 5: 355-364, 1998. [PubMed: 10048485] [Full Text: https://doi.org/10.1093/dnares/5.6.355]
Tompkins, V., Hagen, J., Zediak, V. P., Quelle, D. E. Identification of novel ARF binding proteins by two-hybrid screening. Cell Cycle 5: 641-646, 2006. [PubMed: 16582619]