Alternative titles; symbols
HGNC Approved Gene Symbol: PRAC2
Cytogenetic location: 17q21.32 Genomic coordinates (GRCh38) : 17:48,718,666-48,724,758 (from NCBI)
By database analysis of the genomic region surrounding the PRAC gene (609819), Olsson et al. (2003) identified a cluster of sequence-homologous ESTs derived solely from human prostate cDNA libraries. Using these EST sequences and 5-prime RACE, they assembled a full-length cDNA, which they designated PRAC2. Using constructs expressing each of 3 possible open reading frames expressed in human embryonic kidney 293T cells followed by Western analysis, Olsson et al. (2003) determined that PRAC2 encodes a 14-kD protein, which corresponds to use of the second ORF, with a deduced 90-amino acid sequence and a predicted molecular mass of 10.5 kD. Northern blot analysis detected a 500- to 600-bp transcript in prostate. PCR analysis of 24 tissues detected highest PRAC2 expression in prostate followed by testis; lower expression was detected in placenta, muscle, colon, peripheral blood lymphocytes, and skin. Weak expression was also detected in 2 prostate cancer cell lines. RNA dot-blot analysis of 61 adult and fetal tissues confirmed expression in prostate, some regions of large bowel, and testis. Olsson et al. (2003) concluded that, like PRAC, PRAC2 displays restricted tissue expression with predominant expression in prostate, rectum, and testis. Immunofluorescence and cell fractionation studies localized PRAC2 primarily to the nucleus in NIH3T3 cells with a granular pattern within the cytoplasm as well.
Olsson et al. (2003) determined that the PRAC2 gene contains 2 exons and is transcribed in the opposite orientation to the adjacent PRAC gene.
By genomic sequence analysis, Olsson et al. (2003) mapped the PRAC2 gene (HOXBAS5) to chromosome 17q21 between the HOXB13 (604607) and PRAC genes.
Olsson, P., Motegi, A., Bera, T. K., Lee, B., Pastan, I. PRAC2: a new gene expressed in human prostate and prostate cancer. Prostate 56: 123-130, 2003. [PubMed: 12746837] [Full Text: https://doi.org/10.1002/pros.10185]