Alternative titles; symbols
HGNC Approved Gene Symbol: ZKSCAN5
Cytogenetic location: 7q22.1 Genomic coordinates (GRCh38) : 7:99,504,662-99,534,700 (from NCBI)
By sequencing clones obtained from a brain cDNA library, Nagase et al. (1999) cloned ZKSCAN5, which they designated KIAA1015. The transcript contains a repetitive element in its 3-prime UTR, and the deduced protein contains 839 amino acids. RT-PCR ELISA detected low to moderate expression in all tissues and specific brain regions examined.
By screening a fetal cartilage cDNA library with a degenerate probe corresponding to the Kruppel zinc finger motif, followed by screening amniocyte and testis cDNA libraries, Dreyer et al. (1999) obtained a full-length cDNA encoding ZKSCAN5, which they called ZFP95. The deduced 839-amino acid protein contains an N-terminal SCAN box, followed by a KRAB A domain and a C-terminal zinc finger domain. Dreyer et al. (1999) also identified 2 alternatively spliced transcripts, one encoding a protein lacking the KRAB A domain, and the other encoding a protein lacking the KRAB A domain and an additional 26 amino acids. Northern blot analysis detected 4.2- and 4.6-kb transcripts in all tissues examined, with highest expression in testis. A 3.8-kb transcript was also detected in adult testis.
Weissig et al. (2003) cloned 3 cDNAS for mouse Zfp95. The longest deduced protein contains 819 amino acids and has 12 zinc finger domains in its C-terminal half. Northern blot analysis detected highest Zfp95 expression in testis, with lower levels in brain, heart, kidney, liver, tongue, lung, and striated muscle. A second transcript was expressed exclusively in testis. Expression of the testis-specific Zfp95 transcript in isolated germ cells was highest in pachytene spermatocytes, followed by round spermatids. Mice with the recessive atrichosis (at) mutation are almost hairless and sterile, with small gonads that contain few germ cells. Weissig et al. (2003) found homozygous at mutant mice lacked expression of the testis-specific Zfp95 transcript, whereas the more widely expressed Zfp95 transcript was expressed in testis, epididymis, seminal vesicle, and prostate. They concluded that Zfp95 shows differential splicing in meiotic and postmeiotic germ cells only.
Dreyer et al. (1999) determined that the ZKSCAN5 gene contains 7 exons.
By radiation hybrid analysis, Nagase et al. (1999) mapped the ZKSCAN5 gene to chromosome 7. Using radiation hybrid analysis and FISH, Dreyer et al. (1999) mapped the ZKSCAN5 gene to chromosome 7q22.
Weissig et al. (2003) mapped the mouse Zkscan5 gene to chromosome 5.
Dreyer, S. D., Zheng, Q., Zabel, B., Winterpacht, A., Lee, B. Isolation, characterization, and mapping of a zinc finger gene, ZFP95, containing both a SCAN box and an alternatively spliced KRAB A domain. Genomics 62: 119-122, 1999. [PubMed: 10585779] [Full Text: https://doi.org/10.1006/geno.1999.5981]
Nagase, T., Ishikawa, K., Suyama, M., Kikuno, R., Hirosawa, M., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 6: 63-70, 1999. [PubMed: 10231032] [Full Text: https://doi.org/10.1093/dnares/6.1.63]
Weissig, H., Narisawa, S., Sikstrom, C., Olsson, P. G., McCarrey, J. R., Tsonis, P. A., Del Rio-Tsonis, K., Millan, J. L. Three novel spermatogenesis-specific zinc finger genes. FEBS Lett. 547: 61-68, 2003. [PubMed: 12860387] [Full Text: https://doi.org/10.1016/s0014-5793(03)00669-0]