Alternative titles; symbols
HGNC Approved Gene Symbol: NOP14
Cytogenetic location: 4p16.3 Genomic coordinates (GRCh38) : 4:2,937,936-2,963,406 (from NCBI)
NOP14 plays a role in the processing of the pre-18S rRNA and small ribosomal subunit assembly (Liu and Thiele, 2001).
By screening human brain cDNA libraries, followed by RT-PCR and database analysis, Hadano et al. (1998) isolated a partial NOP14 cDNA, which they called RES4-25. The deduced protein contains 718 amino acids. Northern blot analysis of human tissues detected a 3.8-kb transcript with strong expression in skeletal muscle and liver, moderate expression in pancreas, heart, and kidney, and weak expression in brain, placenta, and lung.
By yeast 2-hybrid screen of a yeast genomic library using Emg1 (611531) as bait, followed by PCR of a Jurkat cell cDNA library, Liu and Thiele (2001) cloned a partial NOP14 cDNA, which they called NOP14. Subcellular fractionation detected yeast Nop14 in the nuclear fraction, and immunofluorescence microscopy localized Nop14 to the nucleolus.
By yeast 2-hybrid analysis, coimmunoprecipitation, and deletion mapping, Liu and Thiele (2001) showed that the central domain (amino acids 170 to 469) of yeast Nop14 interacted with Emg1 and that mouse Emg1 and human NOP14 interacted. Emg1 localized to both nuclear and cytoplasmic fractions in the presence of Nop14 but to the cytoplasmic fraction only in Nop14-depleted cells. Pulse-chase labeling of ribosomal RNA transcript processing in Nop14-depleted yeast showed a processing defect characterized by absence of the 18S rRNA species and its 20S precursor and normal levels of 25S rRNA, similar to the phenotype observed in Emg1 conditional mutant yeast. Sucrose density gradient profiling of cellular ribosomes and polysome runoff strains found significantly reduced levels of 40S subunits compared to 60S. Liu and Thiele (2001) concluded that Nop14 and Emg1 act in the same pathway for 18S rRNA maturation and for 40S ribosome production.
Hadano et al. (1998) determined that the NOP14 gene contains at least 16 exons.
By genomic sequence analysis, Hadano et al. (1998) mapped the NOP14 gene to chromosome 4p16.3.
Hadano, S., Ishida, Y., Ikeda, J.-E. The primary structure and genomic organization of five novel transcripts located close to the Huntington's disease gene on human chromosome 4p16.3. DNA Res. 5: 177-186, 1998. [PubMed: 9734812] [Full Text: https://doi.org/10.1093/dnares/5.3.177]
Liu, P. C. C., Thiele, D. J. Novel stress-responsive genes EMG1 and NOP14 encode conserved, interacting proteins required for 40S ribosome biogenesis. Molec. Biol. Cell 12: 3644-3657, 2001. [PubMed: 11694595] [Full Text: https://doi.org/10.1091/mbc.12.11.3644]