Entry - *611643 - ZINC FINGER PROTEIN WITH KRAB AND SCAN DOMAINS 4; ZKSCAN4 - OMIM

 
 
* 611643

ZINC FINGER PROTEIN WITH KRAB AND SCAN DOMAINS 4; ZKSCAN4


Alternative titles; symbols

ZINC FINGER PROTEIN 307; ZNF307


HGNC Approved Gene Symbol: ZKSCAN4

Cytogenetic location: 6p22.1   Genomic coordinates (GRCh38) : 6:28,241,697-28,259,211 (from NCBI)


▼ TEXT

See 603971 for background information on zinc finger genes.

▼ Cloning and Expression

Li et al. (2007) cloned the zinc finger gene ZKSCAN4, which they designated ZNF307, from human embryonic heart cDNA. The deduced 545-amino acid protein, which is evolutionarily conserved, has a calculated molecular mass of 61.6 kD. It contains a SCAN, or LER (leucine-rich region), domain, a KRAB domain, and 7 C2H2 zinc finger motifs. Northern blot analysis detected a 2.4-kb ZKSCAN4 transcript in adult heart, brain, placenta, lung, and kidney, as well as in embryonic (17-week) heart, brain, skeletal muscle, small intestine, and liver. An EGFP-ZKSCAN4 fluorescent fusion construct showed that the protein localizes to the nucleus.


▼ Gene Function

Using a yeast 2-hybrid system, Li et al. (2007) found that ZKSCAN4 acts as a transcriptional repressor. Further studies demonstrated that full-length ZKSCAN4 suppressed p53 transcription (191170) and that this effect was lessened by siRNA knockdown of ZKSCAN4 transcription. As expected from the known interaction of p53 and p21 (190020), ZKSCAN4 suppressed p21 transcription, which was reversed with siRNA knockdown of ZKSCAN4. Based on experiments showing that overexpression of ZKSCAN4 upregulated expression of the MDM2 (164785) and EP300 (602700) genes, Li et al. (2007) proposed that ZKSCAN4 may suppress the p53-p21 pathway by activating MDM2 and EP300 expression and by inducing degradation of p53 protein.


▼ Gene Structure

Li et al. (2007) determined that the ZKSCAN4 gene contains 5 exons and spans approximately 76.3 kb.


▼ Mapping

By genomic sequence analysis, Li et al. (2007) mapped the ZKSCAN4 gene to chromosome 6p21.33-p21.31.


▼ REFERENCES

  1. Li, J., Wang, Y., Fan, X., Mo, X., Wang, Z., Li, Y., Yin, Z., Deng, Y., Luo, N., Zhu, C., Liu, M., Ma, Q., Ocorr, K., Yuan, W., Wu, X. ZNF307, a novel zinc finger gene suppresses p53 and p21 pathway. Biochem. Biophys. Res. Commun. 363: 895-900, 2007. [PubMed: 17910948, related citations] [Full Text]


Creation Date:
Alan F. Scott : 12/4/2007
Edit History:
mgross : 05/19/2009
carol : 12/4/2007
carol : 12/4/2007

* 611643

ZINC FINGER PROTEIN WITH KRAB AND SCAN DOMAINS 4; ZKSCAN4


Alternative titles; symbols

ZINC FINGER PROTEIN 307; ZNF307


HGNC Approved Gene Symbol: ZKSCAN4

Cytogenetic location: 6p22.1   Genomic coordinates (GRCh38) : 6:28,241,697-28,259,211 (from NCBI)


TEXT

See 603971 for background information on zinc finger genes.

Cloning and Expression

Li et al. (2007) cloned the zinc finger gene ZKSCAN4, which they designated ZNF307, from human embryonic heart cDNA. The deduced 545-amino acid protein, which is evolutionarily conserved, has a calculated molecular mass of 61.6 kD. It contains a SCAN, or LER (leucine-rich region), domain, a KRAB domain, and 7 C2H2 zinc finger motifs. Northern blot analysis detected a 2.4-kb ZKSCAN4 transcript in adult heart, brain, placenta, lung, and kidney, as well as in embryonic (17-week) heart, brain, skeletal muscle, small intestine, and liver. An EGFP-ZKSCAN4 fluorescent fusion construct showed that the protein localizes to the nucleus.


Gene Function

Using a yeast 2-hybrid system, Li et al. (2007) found that ZKSCAN4 acts as a transcriptional repressor. Further studies demonstrated that full-length ZKSCAN4 suppressed p53 transcription (191170) and that this effect was lessened by siRNA knockdown of ZKSCAN4 transcription. As expected from the known interaction of p53 and p21 (190020), ZKSCAN4 suppressed p21 transcription, which was reversed with siRNA knockdown of ZKSCAN4. Based on experiments showing that overexpression of ZKSCAN4 upregulated expression of the MDM2 (164785) and EP300 (602700) genes, Li et al. (2007) proposed that ZKSCAN4 may suppress the p53-p21 pathway by activating MDM2 and EP300 expression and by inducing degradation of p53 protein.


Gene Structure

Li et al. (2007) determined that the ZKSCAN4 gene contains 5 exons and spans approximately 76.3 kb.


Mapping

By genomic sequence analysis, Li et al. (2007) mapped the ZKSCAN4 gene to chromosome 6p21.33-p21.31.


REFERENCES

  1. Li, J., Wang, Y., Fan, X., Mo, X., Wang, Z., Li, Y., Yin, Z., Deng, Y., Luo, N., Zhu, C., Liu, M., Ma, Q., Ocorr, K., Yuan, W., Wu, X. ZNF307, a novel zinc finger gene suppresses p53 and p21 pathway. Biochem. Biophys. Res. Commun. 363: 895-900, 2007. [PubMed: 17910948] [Full Text: https://doi.org/10.1016/j.bbrc.2007.08.180]


Creation Date:
Alan F. Scott : 12/4/2007

Edit History:
mgross : 05/19/2009
carol : 12/4/2007
carol : 12/4/2007



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OMIM® and Online Mendelian Inheritance in Man® are registered trademarks of the Johns Hopkins University.
Copyright® 1966-2024 Johns Hopkins University.

NOTE: OMIM is intended for use primarily by physicians and other professionals concerned with genetic disorders, by genetics researchers, and by advanced students in science and medicine. While the OMIM database is open to the public, users seeking information about a personal medical or genetic condition are urged to consult with a qualified physician for diagnosis and for answers to personal questions.
OMIM® and Online Mendelian Inheritance in Man® are registered trademarks of the Johns Hopkins University.
Copyright® 1966-2024 Johns Hopkins University.
Printed: Nov. 16, 2024