Alternative titles; symbols
HGNC Approved Gene Symbol: ZNF333
Cytogenetic location: 19p13.12 Genomic coordinates (GRCh38) : 19:14,689,787-14,733,742 (from NCBI)
By sequencing clones obtained from a size-fractionated fetal brain cDNA library, Nagase et al. (2001) cloned partial ZNF333, which they designated KIAA1806. RT-PCR ELISA detected moderate expression in adult brain, ovary, fetal liver, fetal brain, heart, lung, and kidney, and low expression in liver, pancreas, spleen, and testis. Cerebellum, caudate nucleus, hippocampus, substantia nigra, and spinal cord showed moderate expression, with low expression in amygdala, corpus callosum, subthalamic nucleus, and thalamus.
By database analysis, followed by RT-PCR of a human peripheral blood lymphocyte cDNA library, and 5-prime RACE of human fetal mRNA, Tian et al. (2002) cloned ZNF333. The deduced 665-amino acid protein has a calculated molecular mass of 75.5 kD and shares 44% amino acid identity with ZNF157 (300024). ZNF333 has 10 C2H2 zinc finger motifs at the C terminus and 2 N-terminal KRAB domains, both containing KRAB A and KRAB B domains. EST database analysis and RT-PCR identified a splice variant with 174 amino acids, only 1 KRAB domain, and lacking zinc finger motifs. Northern blot analysis of human tissues detected a 4.6-kb transcript with strong expression in heart, moderate expression in liver, skeletal muscle, kidney, and pancreas, and low expression in placenta.
By EMSA analysis, Jing et al. (2004) showed that ZNF333 recognized the specific DNA core binding sequence ATAAT. ZNF333 repressed transcription of promoters containing the ATAAT sequence in a luciferase reporter assay.
Tian et al. (2002) determined that the ZNF333 gene contains 14 exons spanning 30 kb.
By genomic sequence analysis, Tian et al. (2002) mapped the ZNF333 gene to chromosome 19p13.1.
Jing, Z., Liu, Y., Dong, M., Hu, S., Huang, S. Identification of the DNA binding element of the human ZNF333 protein. J. Biochem. Molec. Biol. 37: 663-670, 2004. [PubMed: 15607024] [Full Text: https://doi.org/10.5483/bmbrep.2004.37.6.663]
Nagase, T., Nakayama, M., Nakajima, D., Kikuno, R., Ohara, O. Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 8: 85-95, 2001. [PubMed: 11347906] [Full Text: https://doi.org/10.1093/dnares/8.2.85]
Tian, Y., Breedveld, G. J., Huang, S., Oostra, B. A., Heutink, P., Lo, W. H. Y. Characterization of ZNF333, a novel double KRAB domain containing zinc finger gene on human chromosome 19p13.1. Biochim. Biophys. Acta 1577: 121-125, 2002. [PubMed: 12151103] [Full Text: https://doi.org/10.1016/s0167-4781(02)00397-4]