Alternative titles; symbols
HGNC Approved Gene Symbol: ADPGK
Cytogenetic location: 15q24.1 Genomic coordinates (GRCh38) : 15:72,751,294-72,783,758 (from NCBI)
ADPGK (EC 2.7.1.147) catalyzes the ADP-dependent phosphorylation of glucose to glucose-6-phosphate and may play a role in glycolysis, possibly during ischemic conditions (Ronimus and Morgan, 2004).
By database analysis, Ronimus and Morgan (2004) identified human ADPGK. They obtained a mouse Adpgk cDNA derived from mammary tumor and determined by SDS-PAGE that Adpgk has a molecular mass of 51.5 kD. Gel filtration analysis showed that the protein likely is monomeric. Database analysis showed expression in mammalian epithelial, endocrine, lymphatic, muscular, and organ tissues, consistent with a housekeeping function for Adpgk.
Using recombinant mouse Adpgk purified to homogeneity by SDS-PAGE, Ronimus and Morgan (2004) showed that Adpgk exhibited ADP-dependent glucokinase activity and had a bimodal optimal pH at 5.75 to 6.5 and 8.75 to 9.0. Activity was not inhibited by glucose-6-phosphate and other known modulators of glycolytic enzymes; however, high concentrations of glucose significantly inhibited enzyme activity. Mouse Adpgk displayed high specificity for D-glucose, low Km for glucose and ADP, and inhibition by glucose and AMP. Based on these characteristics, Ronimus and Morgan (2004) concluded that Adpgk is a member of the mammalian hexokinase/glucokinase family (see HK1; 142600)
Ronimus and Morgan (2004) determined that the ADPGK gene contains 7 exons and 4 predicted alternative promoters.
By genomic sequence analysis, Ronimus and Morgan (2004) mapped the ADPGK gene to chromosome 15. They mapped the corresponding mouse Adpgk gene to mouse chromosome 9.
Ronimus, R. S., Morgan, H. W. Cloning and biochemical characterization of a novel mouse ADP-dependent glucokinase. Biochem. Biophys. Res. Commun. 315: 652-658, 2004. [PubMed: 14975750] [Full Text: https://doi.org/10.1016/j.bbrc.2004.01.103]