Alternative titles; symbols
HGNC Approved Gene Symbol: CSTF2T
Cytogenetic location: 10q21.1 Genomic coordinates (GRCh38) : 10:51,695,486-51,699,595 (from NCBI)
By sequencing clones obtained from a size-fractionated brain cDNA library, Ishikawa et al. (1998) cloned CSTF2T, which they designated KIAA0689. RT-PCR analysis detected moderate expression in all tissues examined.
Using the RNA-binding domain of mouse Cstr2t as bait, Dass et al. (2002) cloned human CSTF2T from a testis cDNA library. The deduced 616-amino acid protein has a calculated molecular mass of 64.4 kD. It has an N-terminal RNA-binding domain, 9 MEARA/G repeats, and a highly conserved C-terminal domain. Human CSTF2T shares 89.8% amino acid identity with mouse Cstf2t, which contains 630 amino acids and has 8 MEARA/G repeats. Human CSTF2T and CSTF2 (300907) share 74.9% amino acid identity.
Dass et al. (2001) confirmed that the RNA-binding domain of mouse Cstf2t bound RNA.
Dass et al. (2002) determined that the CSTF2T gene is intronless.
By PCR of human/mouse hybrid cell lines and radiation hybrid analysis, Dass et al. (2002) mapped the CSTF2T gene to chromosome 10q22-q23.
Dass et al. (2001) mapped the mouse Cstf2t gene to chromosome 19.
Dass et al. (2007) found that Cstf2t -/- mice were born at the expected mendelian frequency and showed no obvious abnormalities. However, Cstf2t -/- males were infertile and displayed aberrant spermatogenesis, resulting in male infertility that resembled oligoasthenoteratozoospermia. Both Cstft +/- males and Cstft -/- females were fertile. Microarray analysis showed no difference in testis mRNA expression between wildtype and Cstf2t -/- mice at 17 days postpartum, but there were significant differences at 22 and 25 days postpartum. The differences at 22 days postpartum represented mRNAs encoding proteins involved in basic cellular functions, whereas the differences at 25 days postpartum represented mRNAs encoding proteins involved in spermatogenesis functions, thus explaining the infertility phenotype.
Dass, B., McDaniel, L., Schultz, R. A., Attaya, E., MacDonald, C. C. The gene CSTF2T, encoding the human variant CstF-64 polyadenylation protein tau-CstF-64, lacks introns and may be associated with male sterility. Genomics 80: 509-514, 2002. [PubMed: 12408968]
Dass, B., McMahon, K. W., Jenkins, N. A., Gilbert, D. J., Copeland, N. G., MacDonald, C. C. The gene for a variant form of the polyadenylation protein CstF-64 is on chromosome 19 and is expressed in pachytene spermatocytes in mice. J. Biol. Chem. 276: 8044-8050, 2001. [PubMed: 11113135] [Full Text: https://doi.org/10.1074/jbc.M009091200]
Dass, B., Tardif, S., Park, J. Y., Tian, B., Weitlauf, H. M., Hess, R. A., Carnes, K., Griswold, M. D., Small, C. L., MacDonald, C. C. Loss of polyadenylation protein tau-CstF-64 causes spermatogenic defects and male infertility. Proc. Nat. Acad. Sci. 104: 20374-20379, 2007. [PubMed: 18077340] [Full Text: https://doi.org/10.1073/pnas.0707589104]
Ishikawa, K., Nagase, T., Suyama, M., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. DNA Res. 5: 169-176, 1998. [PubMed: 9734811] [Full Text: https://doi.org/10.1093/dnares/5.3.169]