Alternative titles; symbols
HGNC Approved Gene Symbol: TRIM66
Cytogenetic location: 11p15.4 Genomic coordinates (GRCh38) : 11:8,612,040-8,683,231 (from NCBI)
By sequencing clones obtained from a size-fractionated human adult brain cDNA library, Nagase et al. (1997) cloned a partial TRIM66 cDNA, which they designated KIAA0298. RT-PCR of human tissues detected strong expression in testis, thymus, and kidney, moderate expression in prostate and ovary, and low expression in brain, placenta, lung, liver, pancreas, spleen, and small intestine.
By database analysis using mouse Tif1a (TRIM24; 603406) as query, followed by cDNA library screening, RT-PCR, and 5-prime RACE, Khetchoumian et al. (2004) identified human TRIM66, which they called TIF1D, and cloned mouse Trim66. The deduced 1,344-amino acid mouse protein shares similarity with Tif1a, Tif1b (TRIM28; 601742), and Tif1g (TRIM33; 605769). Tif1d contains an N-terminal RBCC (N-terminal RING/B-box/coiled-coil) motif, a C-terminal bromodomain preceded by a PHD finger, and a 39-amino acid central region similar to the HP1 box of Tif1a and Tif1b. Northern blot analysis of mouse tissues detected expression primarily in testis. Immunofluorescence studies localized Tif1d protein to nuclei of postmeiotic, haploid, and elongating spermatids. Western blot analysis detected Tif1d protein in mouse testis from 4 weeks of age onward.
Khetchoumian et al. (2004) showed that Tif1d repressed transcriptional activation of a chimeric activator. Using trichostatin A treatment, they determined that Tif1d repressor activity is mediated by recruitment of deacetylase activity. Yeast 2-hybrid and immunoprecipitation analysis showed that Tif1d interacted with nonhistone chromosomal proteins HP1-alpha (CBX5; 604478), HP1-beta (CBX1; 604511), and HP1-gamma (CBX3; 604477) via the HP1 box, as determined by studies using point mutations in the HP1 box PXVXL motif region. The authors coimmunoprecipitated Tif1d and Hp1-gamma from whole-cell testis extract and used confocal microscopy to localize both proteins to foci distributed throughout the nucleus. Tif1d can form homodimers via the RBCC motif, and Tif1d showed no nuclear receptor-binding activity.
Khetchoumian et al. (2004) determined that the mouse Trim66 gene contains 20 exons spanning 54.4 kb.
By radiation hybrid analysis, Nagase et al. (1997) mapped the TRIM66 gene to chromosome 11.
Khetchoumian, K., Teletin, M., Mark, M., Lerouge, T., Cervino, M., Oulad-Abdelghani, M., Chambon, P., Losson, R. TIF1-alpha, a novel HP1-interacting member of the transcriptional intermediary factor 1 (TIF1) family expressed by elongating spermatids. J. Biol. Chem. 279: 48329-48341, 2004. [PubMed: 15322135] [Full Text: https://doi.org/10.1074/jbc.M404779200]
Nagase, T., Ishikawa, I., Nakajima, D., Ohira, M., Seki, N., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., O'Hara, O. Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. DNA Res. 4: 141-150, 1997. [PubMed: 9205841] [Full Text: https://doi.org/10.1093/dnares/4.2.141]