Alternative titles; symbols
HGNC Approved Gene Symbol: PDCD11
Cytogenetic location: 10q24.33 Genomic coordinates (GRCh38) : 10:103,396,626-103,446,294 (from NCBI)
PDCD11 is a NF-kappa-B (NFKB1; 164011)-binding protein that colocalizes with U3 RNA (180710) in the nucleolus and is required for rRNA maturation and generation of 18S rRNA (Sweet et al., 2003; Sweet et al., 2008).
By sequencing clones obtained from a size-fractionated myeloid leukemia cell line cDNA library, Nagase et al. (1996) cloned PDCD11, which they designated KIAA0185. The deduced protein contains 1,884 amino acids. Northern blot analysis detected moderate expression in testis and low expression in placenta, lung, liver, skeletal muscle, kidney, spleen, thymus, ovary, small intestine, colon, and peripheral blood leukocytes. Expression was weak in heart, brain, and pancreas, and no expression was detected in prostate.
By yeast 2-hybrid screening of a human astrocytoma cell line cDNA library using NFKB1 as bait, Sweet et al. (2003) cloned PDCD11, which they called NFBP. They identified 2 isoforms, which encode a deduced 1,871-amino acid protein and a deduced 131-amino acid NFBP fragment (NFBPF) corresponding to the NFBP C-terminal region, respectively. PDCD11 protein contains 7 S1 RNA-binding domains and a histone acetyltransferase (HAT) repeat and shares 82% and 26% identities with the mouse Pdcd11 and S. cerevisiae Rrp5 orthologs, respectively. Northern blot analysis detected a 5.5-kb PDCD11 transcript in several cell lines and tissues. Immunofluorescence studies localized PDCD11 to granular aggregates in the nucleus and was confirmed to be nucleolar by mass spectrometry of proteins isolated from HeLa cell nucleoli.
Lacana and D'Adamio (1999) cloned mouse Pdcd11, which they called Alg4, using a strategy to identify genes involved in programmed cell death.
Lacana and D'Adamio (1999) demonstrated that mouse Pdcd11 induced transcription of Fas ligand (TNFSF6; 134638) leading to apoptosis.
Sweet et al. (2008) showed that PDCD11 colocalized with and coprecipitated U3 RNA in the nucleolus. Using siRNA knockdown of PDCD11 mRNA, they demonstrated that PDCD11 was required for cleavage and maturation of rRNA precursor and generation of the 18S rRNA species.
Sweet et al. (2003) determined that the PDCD11 gene contains over 35 exons.
By FISH analysis, Sweet et al. (2003) mapped the PDCD11 gene to chromosome 10q24.2-q25.1.
Lacana, E., D'Adamio, L. Regulation of Fas ligand expression and cell death by apoptosis-linked gene 4. Nature Med. 5: 542-547, 1999. [PubMed: 10229231] [Full Text: https://doi.org/10.1038/8420]
Nagase, T., Seki, N., Ishikawa, K., Tanaka, A., Nomura, N. Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1. DNA Res. 3: 17-24, 1996. [PubMed: 8724849] [Full Text: https://doi.org/10.1093/dnares/3.1.17]
Sweet, T., Khalili, K., Sawaya, B. E., Amini, S. Identification of a novel protein from glial cells based on its ability to interact with NF-kappa-B subunits. J. Cell. Biochem. 90: 884-891, 2003. [PubMed: 14624448] [Full Text: https://doi.org/10.1002/jcb.10701]
Sweet, T., Yen, W., Khalili, K., Amini, S. Evidence for involvement of NFBP in processing or ribosomal RNA. J. Cell. Physiol. 214: 381-388, 2008. [PubMed: 17654514] [Full Text: https://doi.org/10.1002/jcp.21204]