Alternative titles; symbols
HGNC Approved Gene Symbol: ZNF296
Cytogenetic location: 19q13.32 Genomic coordinates (GRCh38) : 19:45,071,500-45,076,478 (from NCBI)
By searching an EST database for sequences similar to mouse Znf296, followed by PCR of testis cDNA, Dear (2000) cloned human ZNF296. The deduced 347-amino acid human protein shares 67.3% homology with the 333-amino acid mouse protein. Both contain 6 C2H2-type zinc finger motifs, 6 putative phosphorylation sites, and 7 N-myristoylation sites. RT-PCR analysis of mouse tissues detected highest Znf296 expression in testis and embryonic stem cells. Znf296 was also expressed in bone marrow. In situ hybridization of mouse testis of various developmental stages revealed Znf296 expression in condensing spermatids of adult mouse testis only.
Dear (2000) determined that the ZNF296 gene contains 2 coding exons.
By radiation hybrid analysis, Dear (2000) mapped the ZNF296 gene to chromosome 19. They mapped the mouse Znf296 gene to a region of proximal chromosome 7 that shares homology of synteny with human chromosome 19.
By sequence analysis, Poland et al. (2009) mapped the ZNF296 gene to chromosome 19q13.32.
Poland et al. (2009) reported a 10-year-old boy with acute myeloid leukemia (AML; 601626) subtype M1 and an acquired translocation, t(17;19)(q22;q13.32). The translocation breakpoint on chromosome 17 was in the promoter/enhancer region of the MPO gene (606989), and the breakpoint on chromosome 19 was within the promoter region of the ZNF296 gene, which Poland et al. (2009) called ZNF342. The translocation resulted in a derivative chromosome 17 containing the promoter/enhancer region of the MPO gene fused to the promoter and coding region of the ZNF296 gene, resulting in 90-fold upregulation of ZNF296 expression in the patient's leukemia cells.
Dear, T. N. Cloning, structure, expression analysis, and assignment to mouse chromosome 7 of the gene Zfp296 encoding a zinc finger protein. Mammalian Genome 11: 1037-1039, 2000. [PubMed: 11063263] [Full Text: https://doi.org/10.1007/s003350010182]
Poland, K. S., Shardy, D. L., Azim, M., Naeem, R., Krance, R. A., Dreyer, Z. E., Neeley, E. S., Zhang, N., Qiu, Y. H., Kornblau, S. M., Plon, S. E. Overexpression of ZNF342 by juxtaposition with MPO promoter/enhancer in the novel translocation t(17;19)(q23;q13.32) in pediatric acute myeloid leukemia and analysis of ZNF342 expression in leukemia. Genes Chromosomes Cancer 48: 480-489, 2009. [PubMed: 19255975] [Full Text: https://doi.org/10.1002/gcc.20654]