Alternative titles; symbols
HGNC Approved Gene Symbol: ZNF317
Cytogenetic location: 19p13.2 Genomic coordinates (GRCh38) : 19:9,140,397-9,163,413 (from NCBI)
By sequencing clones obtained from a size-fractionated fetal brain cDNA library, Nagase et al. (2000) cloned ZNF317, which they designated KIAA1588. The transcript contains repetitive elements in its 3-prime end. The deduced 613-amino acid protein shares significant similarity with ZNF184 (602277) and other C2H2-type zinc finger proteins. RT-PCR ELISA detected highest ZNF317 expression in adult brain, kidney, spleen, and ovary. Moderate expression was detected in heart, lung, liver, skeletal muscle, pancreas, testis, fetal brain, fetal liver, and all specific adult brain regions examined.
By RT-PCR of primary cultured human erythroid progenitor cell total RNA, followed by 5-prime and 3-prime RACE, Takashima et al. (2001) cloned ZNF317. RT-PCR revealed 4 ZNF317 splice variants, which Takashima et al. (2001) designated ZNF317-1 through ZNF317-4. ZNF317-2 encodes the full-length 595-amino acid protein, which has a highly conserved N-terminal Kruppel-associated box A (KRAB-A) domain, followed by a divergent KRAB-B domain and 13 Kruppel-type C2H2 zinc fingers in its C-terminal half. ZNF317-1 lacks exon 5, which encodes the KRAB-B domain. ZNF317-3 and ZNF317-4 are identical to ZNF317-1 and ZNF317-2, respectively, except that they contain an insertion of intron 3 that introduces an in-frame premature termination codon. However, ZNF317-3 and ZNF317-4 may be translated from an internal ATG codon corresponding to met86 of ZNF317-2. Northern blot analysis with a nonspecific probe detected a major transcript of about 4.5 kb, predicted to represent ZNF317-1 and ZNF317-2, at variable levels in all tissues examined. A transcript of about 5 kb, predicted to represent ZNF317-3 and ZNF317-4, was detected only in lymphocytes, spleen, and lung. Fluorescent-tagged ZNF317-2 was expressed in the nucleus of transfected COS-7 cells. SDS-PAGE of in vitro transcribed and translated cDNA revealed that ZNF317-1 and ZNF317-2 proteins had apparent molecular masses of 64 and 67 kD, respectively. ZNF317-3 and ZNF317-4 proteins were not detected following in vitro transcription and translation.
Takashima et al. (2001) showed that mitogen stimulation significantly decreased ZNF317 expression in human lymphocytes.
Takashima et al. (2001) determined that the ZNF317 gene contains 7 exons and spans 22 kb. The 3-prime end contains 2 mRNA destabilization signals.
By genomic sequence analysis, Takashima et al. (2001) mapped the ZNF317 gene to chromosome 19p13.
Nagase, T., Kikuno, R., Nakayama, M., Hirosawa, M., Ohara, O. Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 7: 273-281, 2000. [PubMed: 10997877] [Full Text: https://doi.org/10.1093/dnares/7.4.271]
Takashima, H., Nishio, H., Wakao, H., Nishio, M., Koizumi, K., Oda, A., Koike, T., Sawada, K. Molecular cloning and characterization of a KRAB-containing zinc finger protein, ZNF317, and its isoforms. Biochem. Biophys. Res. Commun. 288: 771-779, 2001. [PubMed: 11688974] [Full Text: https://doi.org/10.1006/bbrc.2001.5855]